The direct service will run every Saturday from London St Pancras International and Ashford International starting on July 11 and running until September 5, 2009.
Tickets go on sale on Tuesday, December 23 and are available from £99 return in standard and £249 in Leisure Select.
The journey to Avignon from London St Pancras International takes less than six hours (and less than 5h 30mins from Ashford International) and delivers travellers within a short walk from the ancient city’s walls, surrounding the heart of the historic city.
Simon Montague, of Eurostar, said: "This service is very popular year on year as our travellers greatly appreciate the seamless, comfortable and environmentally friendly journey to Provence.
"Our fares are unchanged since summer 2008 and there are no hidden costs such as surcharges and taxes."
Avignon and over 60 other French destinations are also available all year round by combining a Eurostar journey to Paris and Lille and an onward journey on a French TGV service.
Depart St Pancras International: 7.19am
Depart Ashford International: 7.55am
Arrive Avignon Centre: 2.12pm
Depart Avignon Centre: 4.24pm
Arrive Ashford International: 8.33pm
Arrive St Pancras International: 9.10pm
The Eurostar website.